The Python script 'explain_sam_flags.py' is distributed in '/usr/share/libhtsjdk-java/' as a convenience for the users, but the package does not depend on it. It was converted using 2to3 from Python to Python3. You can install the default Python3 version in Debian with the following command: sudo apt-get install python3 -- Andreas Tille Tue, 17 Apr 2018 15:14:40 +0200